Common Lab Resources in Dr. Leonidas Bleris' Lab
"biological systems made modular"
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Enter keywords for the protocol of your interest. To get the full list of all protocols, use "All".
Protocol Keywords:
Alternatively, you can also search for the protocol of your interest from this drop down list:
17-ODYA Metabolic Labeling
Acyl-Biotin Exchange (ABE) Protocol
Agarose Gel Electrophoresis
Apoptosis Assay
Bacterial Crude Supernatant
Bacterial Protein Expression
Buffer Exchange
Cell Cycle Assay
Collagen Coating
Coomassie Staining
DNA Miniprep
Freezing and Thawing Mammalian Cells
Gel Purification
Genomic DNA preparation using DNeasy Kit
Immunohistochemistry
Immunoprecipitation
Inoculation
Ligation
Passing Mammalian Cells
PCR using NEB Q5 2X High-Fidelity Master Mix
Preparation of Bacterial Growth Broth
Preparation of Bacterial Growth Plates
Preparation of Chemically Competent Cells
Preparation of Mammalian Cell Culture Medium
Preparation of mouse NSC (neural stem cell) and SILAC
Preparation of mouse neuronal cultures
Preparation of Plasmid Glycerol Stock
Quantitative PCR Using KAPA SYBR Kit
Restriction Enzyme Digestion
Reverse Transcription Using QuantiTech RT Kit
RNA Preparation Using RNeasy Kit
SDS-PAGE
siRNA Reverse Transfection
Southern Blot
Transformation (DH5alpha)
Transformation (Homemade Competent Cells)
Transformation (XL10-Gold UltraCompetent Cells)
Transient Transfection using JetPrime